Simultaneous real-time estimation of maximum substrate uptake capacity and yield coefficient in induced microbial cultures

In this work we present a soft sensor to accurately estimate the yield coefficient YX/S and substrate uptake capacity qSmax in cultivation process using offgas measurements nonlinear state observer with an underlying mechanistic model. The structural observability analysis of model showed that both parameters are observable given available measurement information. different simulation scenarios analyzed under which conditions accurate estimation is possible when uncertain. Testing proposed in-silico can be estimated reasonable accuracy depending on parameter sensitivity. Verification developed was carried out induced Escherichia coli cultivations. Besides prediction living biomass, accumulation, decreasing could detected. Therefore, used control cultures prevent overfeeding situations.